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Vitamin K-dependent carboxylation of glutamic acid residues to γ-carboxyglutamic acid in lung microsomes

✍ Scribed by Robert G. Bell


Book ID
115704360
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
615 KB
Volume
203
Category
Article
ISSN
0003-9861

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Direct identification of γ-carboxyglutam
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We report the first direct method for the identification of the vitamin K-dependent Ca2+ binding amino acid, gamma-carboxyglutamic acid (Gla), in the sequencing of proteins. The carboxyl groups on the protein are first converted to methyl esters with methanolic HCl, a procedure that reduces the pola