Direct identification of γ-carboxyglutam
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James R. Cairns; Matthew K. Williamson; Paul A. Price
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Article
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1991
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Elsevier Science
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English
⚖ 430 KB
We report the first direct method for the identification of the vitamin K-dependent Ca2+ binding amino acid, gamma-carboxyglutamic acid (Gla), in the sequencing of proteins. The carboxyl groups on the protein are first converted to methyl esters with methanolic HCl, a procedure that reduces the pola