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Detection of vitamin K-dependent proteins in venoms with a monoclonal antibody specific for γ-carboxyglutamic acid

✍ Scribed by M.A. Brown; B. Hambe; B. Furie; B.C. Furie; J. Stenflo; L.M. Stenberg


Book ID
117227363
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
167 KB
Volume
40
Category
Article
ISSN
0041-0101

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Direct identification of γ-carboxyglutam
✍ James R. Cairns; Matthew K. Williamson; Paul A. Price 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 430 KB

We report the first direct method for the identification of the vitamin K-dependent Ca2+ binding amino acid, gamma-carboxyglutamic acid (Gla), in the sequencing of proteins. The carboxyl groups on the protein are first converted to methyl esters with methanolic HCl, a procedure that reduces the pola