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Visualization of proteins after isoelectric focusing during two-dimensional gel electrophoresis

✍ Scribed by Herbert Jäckle

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
860 KB
Volume
98
Category
Article
ISSN
0003-2697

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✦ Synopsis

A modification of P. H. O'Farrell's (1975,5. Viol. Chrrrr. 259,4007-4021) two-dimensional gel electrophoresis is described.

After isoelectric focusing, the cylindrical gels were fixed and stained with Coomassie brilliant blue R before sodium dodecyl sulfate-slab gel electrophoresis in the second dimension.

The modification does not alter the protein patterns obtained, but provides sharper spots. In addition, bands are made visible before separation in the second dimetnjion.

Moreover, the modification helps to reduce the amount of ampholine in the dye front during electrophoresis.

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