Isoelectric focusing and gradient gel electrophoresis: A two-dimensional technique
β Scribed by K.G. Kenrick; J. Margolis
- Publisher
- Elsevier Science
- Year
- 1970
- Tongue
- English
- Weight
- 930 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A modification of P. H. O'Farrell's (1975,5. Viol. Chrrrr. 259,4007-4021) two-dimensional gel electrophoresis is described. After isoelectric focusing, the cylindrical gels were fixed and stained with Coomassie brilliant blue R before sodium dodecyl sulfate-slab gel electrophoresis in the second di
## Paris equilibrium iSOeleCtI'k focusing in Cader ampholyte- A modified procedure for the two-dimensional electrophoretic analysis of basic polypeptides is described. This method uses isoelectric focusing with carrier ampholytes in the first dimension, and sodium dodecyl sulfate-electrophoresis i
Recent advances in protein sequence analysis now permit the determination of partial N-terminal and internal primary structure from low picomole quantities of protein. The major remaining hurdles to sequence analysis of small amounts of protein are the identification, isolation, and handling of micr