Recent advances in protein sequence analysis now permit the determination of partial N-terminal and internal primary structure from low picomole quantities of protein. The major remaining hurdles to sequence analysis of small amounts of protein are the identification, isolation, and handling of micr
Agarose isoelectric focusing can improve resolution of membrane proteins in the two-dimensional electrophoresis of bacterial proteins
โ Scribed by Pia Altenhofer; Angelika Schierhorn; Beate Fricke
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 606 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0173-0835
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## Paris equilibrium iSOeleCtI'k focusing in Cader ampholyte- A modified procedure for the two-dimensional electrophoretic analysis of basic polypeptides is described. This method uses isoelectric focusing with carrier ampholytes in the first dimension, and sodium dodecyl sulfate-electrophoresis i
## Two-dimensional analysis of membrane proteins with isoelectric focusing in immobilized pH gradients in the first dimension Two-dimensional electrophoresis with isoelectric focusing in immobilized pH gradients in the first dimension was applied to the fractionation of hydrophobic proteins from th