𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Viscosity and temperature effects on the rate of DNA strand separation in alkali

✍ Scribed by Peter F. Davison


Publisher
Wiley (John Wiley & Sons)
Year
1967
Tongue
English
Weight
419 KB
Volume
5
Category
Article
ISSN
0006-3525

No coin nor oath required. For personal study only.

✦ Synopsis


The time required for the completion of the process of strand separation ill X bacteriophage DNA preparations exposed to strongly alkaline conditions in aqueous glycerol solutions was directly proportional to the viscosity of the solvent. This finding s u p ports the idea that the rate-limiting step in the denaturation of high molecular weight DNA is the uncoiling of the strands and in the course of this uncoiling the forces arising from electrostatic and entropic factors must overcome the hydrodynamic viacotis drag of the solvent resisting the translation of the polynucleotide strands.


πŸ“œ SIMILAR VOLUMES


Effect of temperature on the limiting vi
✍ Robert L. Cleland πŸ“‚ Article πŸ“… 1979 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 434 KB πŸ‘ 2 views

## Abstract The decrease in the limiting viscosity number [Ξ·] with temperature __T__ for hyaluronic acid in nonalkaline solution and chondroitin 4‐sulfate in neutral and alkaline solutions may be expressed in terms of the temperature coefficient of the Kratky‐Porod persistence length __a__: __d__ l

The effects of alkali-metal chlorides an
✍ Alfred J. Richard πŸ“‚ Article πŸ“… 1984 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 322 KB πŸ‘ 1 views

## Abstract The effects is solution of the alkali‐metal chlorides on the gel‐like phase of DNA formed in the ultracentrifuge cell have been studied. The polycations, spermidine and spermine, also were shown to affect strongly the swelling pressure of the DNA gel, with evidence for the destabilizati

Independence of length and temperature e
✍ Cheng H. Lee; James G. Wetmur πŸ“‚ Article πŸ“… 1972 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 483 KB πŸ‘ 1 views

The rate of double helix formation by single stranded Poly A plus Poly U, Poly I plus Poly C, Poly G plus Poly C, and T 2 DNA has been investigated as a function of both the length of the reacting strands and temperature. The length dependence of the rate is found to be independent of temperature. A