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Independence of length and temperature effects on the rate of helix formation between complementary ribopolymers

✍ Scribed by Cheng H. Lee; James G. Wetmur

Publisher
Wiley (John Wiley & Sons)
Year
1972
Tongue
English
Weight
483 KB
Volume
11
Category
Article
ISSN
0006-3525

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✦ Synopsis

The rate of double helix formation by single stranded Poly A plus Poly U, Poly I plus Poly C, Poly G plus Poly C, and T 2 DNA has been investigated as a function of both the length of the reacting strands and temperature. The length dependence of the rate is found to be independent of temperature. All of the reactions studied show a rate approximately proportional to the square root of the length of the shorter of the complementary strands. At or about 30°C below the melting temperature the ribopolymers react with about the same rate. This rate is four to five times slower than DNA renaturation rates. The effect of temperature on ribopolymer reaction rates is interpreted in terms of a steady-state model for helix propagation.

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