𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The effects of alkali-metal chlorides and of spermidine and spermine on the swelling pressures of DNA gels in the ultracentrifuge

✍ Scribed by Alfred J. Richard


Publisher
Wiley (John Wiley & Sons)
Year
1984
Tongue
English
Weight
322 KB
Volume
23
Category
Article
ISSN
0006-3525

No coin nor oath required. For personal study only.

✦ Synopsis


Abstract

The effects is solution of the alkali‐metal chlorides on the gel‐like phase of DNA formed in the ultracentrifuge cell have been studied. The polycations, spermidine and spermine, also were shown to affect strongly the swelling pressure of the DNA gel, with evidence for the destabilization of DNA in very dilute spermine, below 10^βˆ’6^ M, and for the collapse of DNA in both spermine and spermidine solutions above 10^βˆ’6^ M.


πŸ“œ SIMILAR VOLUMES


Viscosity and temperature effects on the
✍ Peter F. Davison πŸ“‚ Article πŸ“… 1967 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 419 KB πŸ‘ 2 views

The time required for the completion of the process of strand separation ill X bacteriophage DNA preparations exposed to strongly alkaline conditions in aqueous glycerol solutions was directly proportional to the viscosity of the solvent. This finding s u p ports the idea that the rate-limiting step

DNA electrophoresis in agarose gels: Eff
✍ Randolph L. Rill; Afshin Beheshti; David H. Van Winkle πŸ“‚ Article πŸ“… 2002 πŸ› John Wiley and Sons 🌐 English βš– 190 KB πŸ‘ 2 views

Electrophoretic mobilities of DNA molecules ranging in length from 200 to 48 502 base pairs (bp) were measured in agarose gels with concentrations T = 0.5% to 1.3% at electric fields from E = 0.71 to 5.0 V/cm. This broad data set determines a range of conditions over which the new interpolation equa