The effects of alkali-metal chlorides and of spermidine and spermine on the swelling pressures of DNA gels in the ultracentrifuge
β Scribed by Alfred J. Richard
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1984
- Tongue
- English
- Weight
- 322 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0006-3525
No coin nor oath required. For personal study only.
β¦ Synopsis
Abstract
The effects is solution of the alkaliβmetal chlorides on the gelβlike phase of DNA formed in the ultracentrifuge cell have been studied. The polycations, spermidine and spermine, also were shown to affect strongly the swelling pressure of the DNA gel, with evidence for the destabilization of DNA in very dilute spermine, below 10^β6^ M, and for the collapse of DNA in both spermine and spermidine solutions above 10^β6^ M.
π SIMILAR VOLUMES
The time required for the completion of the process of strand separation ill X bacteriophage DNA preparations exposed to strongly alkaline conditions in aqueous glycerol solutions was directly proportional to the viscosity of the solvent. This finding s u p ports the idea that the rate-limiting step
Electrophoretic mobilities of DNA molecules ranging in length from 200 to 48 502 base pairs (bp) were measured in agarose gels with concentrations T = 0.5% to 1.3% at electric fields from E = 0.71 to 5.0 V/cm. This broad data set determines a range of conditions over which the new interpolation equa