Effect of gelation conditions on the gel structure and resolving power of agarose-based DNA sequencing gels

DNA electrophoresis in agarose gels: Eff

DNA electrophoresis in agarose gels: Effects of field and gel concentration on the exponential dependence of reciprocal mobility on DNA length

✍ Randolph L. Rill; Afshin Beheshti; David H. Van Winkle 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 190 KB 👁 2 views

Electrophoretic mobilities of DNA molecules ranging in length from 200 to 48 502 base pairs (bp) were measured in agarose gels with concentrations T = 0.5% to 1.3% at electric fields from E = 0.71 to 5.0 V/cm. This broad data set determines a range of conditions over which the new interpolation equa