The viscosity profiles of four different IgG 1 molecules were studied as a function of concentration at pH 6.0. At high concentrations, MAb-H and -A showed significantly higher viscosities as compared to MAb-G and -E. Zeta Potential (j) measurements showed that all the IgG 1 molecules carried a net
Virus filtration of high-concentration monoclonal antibody solutions
✍ Scribed by Bruno F. Marques; David J. Roush; Kent E. Göklen
- Publisher
- American Institute of Chemical Engineers
- Year
- 2009
- Tongue
- English
- Weight
- 287 KB
- Volume
- 25
- Category
- Article
- ISSN
- 8756-7938
- DOI
- 10.1002/btpr.177
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
The purpose of this work was to understand the viscosity behavior of highconcentration monoclonal antibody (mAb) solutions in a wide range of solution conditions and generate guidelines helpful to formulate products with manageable viscosity. The zeta potential and effective isoelectric point (pI) w
## Abstract There is concern that shear could cause protein unfolding or aggregation during commercial biopharmaceutical production. In this work we exposed two concentrated immunoglobulin‐G1 (IgG1) monoclonal antibody (mAb, at >100 mg/mL) formulations to shear rates between 20,000 and 250,000 s^−1