Variations in the protein pattern of ehrlich ascites tumor cell polyribosomes
โ Scribed by Alap R. Subramanian; E.C. Henshaw
- Book ID
- 115746839
- Publisher
- Elsevier Science
- Year
- 1978
- Weight
- 507 KB
- Volume
- 520
- Category
- Article
- ISSN
- 0005-2787
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Proteins present in messenger ribonucleoprotein particles were labeled with [35S]-methionine in Ehrlich ascites tumor cells in which synthesis of new ribosomes was inhibited. Poly(A)-protein complexes were isolated from free and membrane-bound polyribosomes by sucrose gradient centrifugation and aff
Data from isotopic uptake experiments were used to measure the kinetics of labelling of cellular phosphate, ATP and ADP in the Ehrlich ascites tumor cell. The results show that steady state phosphate exchange flux was 0.333 % 0.052 (S.E.) pmoles per lo' cells per hour at 37", and that the specific a
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