✦ LIBER ✦

The transport of chloride in ehrlich ascites tumor cells

✍ Scribed by Charles Levinson; Mitchel L. Villereal

Publisher: John Wiley and Sons
Year: 1976
Tongue: English
Weight: 785 KB
Volume: 88
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040880207

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✦ Synopsis

Abstract

The steady state transport and distribution of chloride between the intracellular and extracellular phases was investigated when the extracellular chloride concentration was varied by isosmotic replacement with nitrate, bromide and acetate. The results of these experiments show that chloride transport, measured by uptake of ^36^Cl, is sensitive to the replacement anion. In the presence of nitrate, chloride transport is a linear function of the extracellular chloride concentration. The relationship between chloride transport and extracellular chloride in the presence of bromide is concave upward which suggests that this anion inhibits chloride movement. However, when acetate replaces chloride, the relationship between chloride transport and extracellular chloride is concave downward. The chloride distribution ratio of cells incubated in 145–155 mM chloride medium is 0.386 and is not effected by the replacement of chloride with nitrate, bromide or acetate.

These findings are consistent with the assertion that chloride transport is composed of two parallel pathways, a diffusional plus a saturating, mediated component. Of the total chloride flux (9.1 mmoles Cl‐/kg dry weight per minute) measured in chloride medium (145–155 mM Cl ‐), the mediated component represents 40% and the diffusional component 60%.

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