Chloride and sulfate transport in ehrlich ascites tumor cells: Evidence for a common mechanism

✍ Scribed by Charles Levinson

• Publisher: John Wiley and Sons
• Year: 1978
• Tongue: English
• Weight: 982 KB
• Volume: 95
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1040950104

✦ Synopsis

The effects of phloretin, H,DIDS (4,4'-diisothiocyano-1,2-diphenylethane-2,2'-disulfonate) and SO4-' on anion transport in Ehrlich ascites tumor cells was studied in an effort to determine whether C1-and share a common transport mechanism. Sulfate, in the presence of constant extracellular C1-(100 mM), reduces C1-self-exchange by 43% (40 mM and C1--S04-2 exchange by 36% (25mM Cl-/O Phloretin blocks without delay and to the same extent the self-exchange of both C1-and SO4-'. For example, at M phloretin, anion transport is inhibited 28% which increases to 78% a t 5 x M. Reversibly bound H,DIDS also inhibits the self-exchange of both C1-and However, at all H,DIDS concentrations tested (0.5 -10 X M) SO4-' transport was far more susceptible to inhibition than that of C1-. HzDIDS when irreversibly bound to the cell inhibits SO4-' but not C1-transport.

The results of these experiments are consistent with the postulation that both C1-and are transported by a common mechanism possessing two reactive sites.