✦ LIBER ✦

Calcium transport and distribution in ehrlich mouse ascites tumor cells

✍ Scribed by Dr. Charles Levinson; Leslie E. Blumenson

Publisher: John Wiley and Sons
Year: 1970
Tongue: English
Weight: 693 KB
Volume: 75
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040750212

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Data from isotopic uptake experiments were used to measure calcium fluxes and compartment sizes in ascites tumor cells. The data were analyzed with two kinetic models, A and B. In 80% of the experiments model A, which is based on one exchangeable calcium compartment, was rejected in favor of Model B, which predicts two exchangeable compartments. A statistical evaluation of the model's performance, when fit to the experimental data was used to select between the two models.

The results show that calcium was distributed between three cellular compartments in the ratio, non‐exchangeable (88%): rapidly exchanging (7%): slowly exchanging (5%). The undirectional fluxes suggested that calcium transport could be described as a series system with the temporal sequence: environment ⇄ rapidly exchanging ⇄ slowly exchanging.

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