✦ LIBER ✦

Characterization of poly(a)-protein complexes isolated from free and membrane-bound polyribosomes of Ehrlich ascites tumor cells

✍ Scribed by Dick B. Janssen; Anda D. Counotte-Potman; Walther J. Venrooij

Publisher: Springer
Year: 1976
Tongue: English
Weight: 535 KB
Volume: 3
Category: Article
ISSN: 0301-4851
DOI: 10.1007/bf00357213

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✦ Synopsis

Proteins present in messenger ribonucleoprotein particles were labeled with [35S]-methionine in Ehrlich ascites tumor cells in which synthesis of new ribosomes was inhibited. Poly(A)-protein complexes were isolated from free and membrane-bound polyribosomes by sucrose gradient centrifugation and affinity chromatography on oligo(dT)-cellulose. Both classes of Poly(A)-protein particles contain a poly(A) chain of about 70 adenyl residues and a protein with a molecular weight of 76000 attached to it.

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