✦ LIBER ✦

Phosphorus incorporation in the ehrlich ascites tumor cell

✍ Scribed by Charles Levinson; Edward D. Gordon Jr.

Publisher: John Wiley and Sons
Year: 1971
Tongue: English
Weight: 606 KB
Volume: 78
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040780213

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✦ Synopsis

Data from isotopic uptake experiments were used to measure the kinetics of labelling of cellular phosphate, ATP and ADP in the Ehrlich ascites tumor cell. The results show that steady state phosphate exchange flux was 0.333 % 0.052 (S.E.) pmoles per lo' cells per hour at 37", and that the specific activity of phosphate was the same as P, ATP. Metabolic inhibition reduced the phosphate flux by 30-50%. A model, based on oxidative phosphorylation and the adenylate kinase reaction is used to interpret the labelling sequence of P, ATP and P, ADP, and its dependence on P, ATE' .

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