Variation in the storage proteins of peas in relation to sulphur amino-acid content

✍ Scribed by D. Roy Davies

• Publisher: Springer
• Year: 1976
• Tongue: English
• Weight: 430 KB
• Volume: 25
• Category: Article
• ISSN: 0014-2336
• DOI: 10.1007/bf00041611

✦ Synopsis

A cellulose acetate electrophoretic technique has been used to characterise the storage proteins present in pea seeds in order to try and determine the proportions of proteins present which are enriched in sulphur amino-acids. Some of the eight varieties examined were shown to differ in their proportions of the various storage proteins. When the proteins were separated and estimated quantitatively and also characterised by their sub-unit composition, it was shown that cellulose acetate electrophoresis of crude protein extracts or of whole globulins did not give the degree of resolution required. The procedures which have to be undertaken in order to obtain this information are considered, and the difficulties of incorporating them into a plant breeding programme discussed.