Using on-line solid phase extraction for flow-injection spectrophotometric determination of salbutamol

✍ Scribed by Dalibor Šatı́nský; Rolf Karlı́ček; Antonı́n Svoboda

• Publisher: Elsevier Science
• Year: 2002
• Tongue: English
• Weight: 229 KB
• Volume: 455
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/s0003-2670(01)01586-0

✦ Synopsis

In the proposed procedure, the determination of salbutamol with Folin-Ciocalteau reagent (FC) using a flow injection analysis technique (FIA) with spectrophotometric detection at 750 nm is described. The lab-made FIA system consisted of a peristaltic pump Gilson Minipulse 3 equipped with Tygon tubes, double 6-port external Vici Valco sample injector and S 2000/SAD500 fiber optic spectrophotometer. It was controlled by a PC with use of originally compiled LabVIEW ®supported software containing the mathematical library with various statistical functions for off-line data evaluation. Concentration, volume of reagents and flow rate were optimised by a simplex method. The proposed system was used for the direct determination of salbutamol sulphate in the tablets and the human urine without preliminary pre-treatment of the sample. The negative effect of interfering substances (excipients of the tablets and matrix of the urine) is overcome by a solid phase extraction (SPE), when salbutamol is adsorbed on the solid phase in the microcolumn, which is integrated directly into the flow system. Pre-treatment of the sample takes place directly in the flowing stream. The sample throughput without carryover of on-line SPE was 60-80 samples per hour. With the SPE column (Baker-carboxylic acid), salbutamol was determined in the linear range from 1 to 15 g ml -1 (R.S.D. = 1.2%), with detection limit (3σ ) 0.1 g ml -1 and a frequency of 40-60 samples per hour in the water solutions. The salbutamol was determined in the linear range from 2 to 20 g ml -1 (R.S.D. = 1.7%), with detection limit (3σ ) 1 g ml -1 and a frequency of 30 samples per hour in the samples of the human urine.