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Use of tetraphenylethylene in quantitative GLC of hyoscyamine and scopolamine

✍ Scribed by Bang-Ling Wu Chu; Edward S. Mika

Publisher
John Wiley and Sons
Year
1970
Tongue
English
Weight
309 KB
Volume
59
Category
Article
ISSN
0022-3549

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✦ Synopsis

Hyoscyamine and scopolamine as free bases were simultaneously quantitated by GLC, using tetraphenylethylene as an internal standard. A linear relationship was found between the ratios of the integrated peak areas of alkaloid to the internal standard and the actual weight ratios of alkaloid to the internal standard for both hyoscyamine and scopolamine. Pure compounds and extracts from Hyoscyamus niger Linne powder were analyzed. The combined precision of the extraction procedure of plant materials with the GLC procedure was determined.

Keyphrases 0 Tetraphenylethylene, internal standard-quantitative GLC of hyoscyamine, scopolamine 0 GLC, hyoscyamine, scopolamine, quantitative-tetraphenylethylene, internal standard Hyoscyamine-simultaneous GLC quantification with scopolamine using tetraphenylethylene as internal standard 0

Scopolamine-simultaneous GLC quantification with hyoscyamine using tetraphenylethylene as internal standard

πŸ“œ SIMILAR VOLUMES

Microdetermination of hyoscyamine and sc
Microdetermination of hyoscyamine and scopolamine in mixtures
✍ Sawsan El-Masry; Saleh A. H. Khalil πŸ“‚ Article πŸ“… 1973 πŸ› John Wiley and Sons 🌐 English βš– 293 KB

Following the acid-dye technique, hyoscyamine (or atropine) could he selectively determined in the presence of scopolamine, using bromcresol purple at pH 6.6. Total alkaloids were determined using bromthymol blue at pH 5.6. By referring to calibration curves of the two alkaloids with the appropriate