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Biosynthesis of hyoscyamine and scopolamine in Datura stramonium

✍ Scribed by E. W. Underhill; H. W. Youngken Jr.


Publisher
John Wiley and Sons
Year
1962
Tongue
English
Weight
476 KB
Volume
51
Category
Article
ISSN
0022-3549

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Following the acid-dye technique, hyoscyamine (or atropine) could he selectively determined in the presence of scopolamine, using bromcresol purple at pH 6.6. Total alkaloids were determined using bromthymol blue at pH 5.6. By referring to calibration curves of the two alkaloids with the appropriate

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Hyoscyamine and scopolamine as free bases were simultaneously quantitated by GLC, using tetraphenylethylene as an internal standard. A linear relationship was found between the ratios of the integrated peak areas of alkaloid to the internal standard and the actual weight ratios of alkaloid to the in