Use of dye-labeled protein as spectrophotometric assay for protein precipitants such as tannin
β Scribed by Thomas N. Asquith; Larry G. Butler
- Publisher
- Springer
- Year
- 1985
- Tongue
- English
- Weight
- 511 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0098-0331
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π SIMILAR VOLUMES
Fluorescamine is a useful reagent in monitoring protein-DNA interactions only if a convenient method of separating the complex from free protein is available. Sedimentation of the complex provides such a method at least in the case of histone-like proteins capable of extensive interaction with DNA.
Alkaline hydrolysis of protein followed by reaction with o-phthalaldehyde has permitted the determination of less than 10 ng of protein fluorometrically. When intact proteins were analyzed with o-phthalaldehyde the reaction was less sensitive. This reaction has been applied to analysis of the protei