Using periodate-oxidized nucleotide as affinity label for the nucleotide site of proteins
โ Scribed by Chin-Chun Lin; Gu-Gang Chang
- Publisher
- Springer
- Year
- 1993
- Tongue
- English
- Weight
- 520 KB
- Volume
- 12
- Category
- Article
- ISSN
- 1573-4943
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๐ SIMILAR VOLUMES
A novel approach for identification of adenine and guanine nucleotide-binding proteins in permeabilized cells is described. Cells were incubated for various periods with \(\alpha-{ }^{32} \mathrm{P}\)-labeled nucleotides and then briefly treated with periodate. Condensation products formed in situ b
The mass spectrometric behaviour of six cyclic nucleotide analogues which activate cyclic AMP-dependent protein kinase was studied by positive-ion fast-atom bombardment (FAB) and collision-induced dissociation (CID) mass-analysed ion kinetic energy (MIKE) spectrometry. The compounds studied were 1,N