Use of 5′-[p-(Fluorosulfonyl)benzoyl] Guanosine as an Affinity Probe for the Guanine Nucleotide-Binding Site of Transducin

A three-step procedure for the isolation

A three-step procedure for the isolation of peptides derived from adenine nucleotide binding sites of enzymes labeled with p-fluorosulfonyl [14C]benzoyl-5′-adenosine

✍ Fred S. Esch; William S. Allison 📂 Article 📅 1979 🏛 Elsevier Science 🌐 English ⚖ 334 KB

A simple three-step procedure is described for the purification of a labeled peptide from a tryptic digest of the P-subunit of the F,-ATPase after the enzyme had been inactivated with p-fluorosulfonyl-[14C]benzoyl-5'-adenosine. The procedure involves: (1) anion-exchange chromatography of a tryptic