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Fluorometric assays in the study of nucleic acid-protein interactions: II. The use of fluorescamine as a reagent for proteins

✍ Scribed by JoséV. Castell; Angel Pestaña; Ricardo Castro; Roberto Marco

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
574 KB
Volume
90
Category
Article
ISSN
0003-2697

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✦ Synopsis

Fluorescamine is a useful reagent in monitoring protein-DNA interactions only if a convenient method of separating the complex from free protein is available. Sedimentation of the complex provides such a method at least in the case of histone-like proteins capable of extensive interaction with DNA. This approach is therefore complementary to the filter binding assay. When the interaction of protein and DNA is compared by both methods. a clear-cut distinction between two steps is obtained: (i) a nucleation step that can be measured by the filter binding assay; and (ii) the cooperative growth of the complex that can only be measured by the sedimentation assay. The method is also useful to detect small amounts of protease contamination in DNA preparations.

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