๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Use of CE for the determination of binding constants

โœ Scribed by Chunxia Jiang; Daniel W. Armstrong


Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
160 KB
Volume
31
Category
Article
ISSN
0173-0835

No coin nor oath required. For personal study only.

โœฆ Synopsis


Abstract

In the past two decades, CE has been used frequently for the measurement of apparent binding/association constants. CE has numerous advantages, including short analysis times, low sample consumption, simplicity of operation, etc. This review provides a fundamental introduction to binding theory and then summarizes recent applications and advances in the field of CEโ€based methods for the evaluation of molecular association. The time period for this survey is from 2002 (when our previous review was published) to the present.


๐Ÿ“œ SIMILAR VOLUMES


Fluorescence displacement method for the
โœ Petr Kuzmiฤ; Marcia L. Moss; James L. Kofron; Daniel H. Rich ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 473 KB

The equilibrium constant for the binding of a spectroscopically invisible ligand to its protein receptor can be determined in a competition experiment, by using a structural analog that contains a reporter group (fluorophor). A novel mathematical treatment of the multiple equilibria allows the analy

New Approach for Determination of Macros
โœ H Gharibi; M.A Safarpour; A.A Rafati ๐Ÿ“‚ Article ๐Ÿ“… 1999 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 97 KB

A new method has been developed for determining macroscopic binding constants of a few surfactants to some macromolecules. Then, the binding of ionic surfactants (cetyltrimethyl ammonium bromide, tetradecyltrimethyl ammonium bromide, and sodium tetradecyl sulfate) [STS]) to macromolecules (bovine se

A Simple Method for Determining Kinetic
โœ Z.X. Wang ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 467 KB

A general procedure is described for determining the kinetic constants of the slow, tight-binding inhibition of enzyme-catalyzed reactions by analyzing the data of initial and steady-state rate. All unknown parameters can be determined from several simple, sequential calculations. This method is sim