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New Approach for Determination of Macroscopic Binding Constants of Ligands to Macromolecules

✍ Scribed by H Gharibi; M.A Safarpour; A.A Rafati

Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
97 KB
Volume
219
Category
Article
ISSN
0021-9797

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✦ Synopsis

A new method has been developed for determining macroscopic binding constants of a few surfactants to some macromolecules. Then, the binding of ionic surfactants (cetyltrimethyl ammonium bromide, tetradecyltrimethyl ammonium bromide, and sodium tetradecyl sulfate) [STS]) to macromolecules (bovine serum albumin, human serum albumin, and polyvinyl pyrollidone was investigated. The binding constants and binding isotherms were calculated using this new method. Experimental data were obtained through a potentiometry technique. Finally, the results obtained were compared with the results obtained based on the Wyman binding potential concept.

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