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Ligand binding to macromolecules: Determination of binding parameters by combined use of ligand buffers and flow dialysis; application to calcium-binding proteins

โœ Scribed by Jacques Haiech; Bernard Vallet; Robert Aquaron; Jacques G. Demaille


Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
438 KB
Volume
105
Category
Article
ISSN
0003-2697

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Low-Affinity Binding Determined by Titra
โœ Yan-Ling Zhang; Zhong-Yin Zhang ๐Ÿ“‚ Article ๐Ÿ“… 1998 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 187 KB

A competition-based method is used for the determination of the thermodynamic parameters for a low-affinity ligand binding reaction by isothermal titration calorimetry. This method is based on the coupling of a high-affinity ligand to the binding of the low-affinity ligand. Results are presented for