Tritium labeling of steroids by microwave discharge activation of tritium gas

✍ Scribed by W. H. Chiu; C. T. Peng

• Publisher: John Wiley and Sons
• Year: 1979
• Tongue: French
• Weight: 446 KB
• Volume: 16
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.2580160413

✦ Synopsis

Pharmacy, U n i v e r s i t y o f C a l i f o r n i a , San Francisco, C a l i f o r n i a 94143 SUNWARY S t e r o i d s w i t h d i a z o and a z i d o f u n c t i o n a l groups were l a b e l e d w i t h t r i t i u m atoms by microwave d i scharge a c t i v a t i o n o f t r i t i u m g a s .

The s p e c i f i c a c t i v i t y o f t h e p r o d u c t s was i n c r e a s e d by maximizing t h e exposure s u r f a c e u s i n g g l a s s f i b e r paper f o r d i s p e r s i o n s reducing t h e amount of sample t o be exposed, and i n c r e a s i n g t r i t i u m p r e s s u r e . A 500 f o l d i n c r e a s e i n s p e c i f i c a c t i v i t y was achieved i n d i spersed sample as compared t o t h e s o l i d . Addition o f t r i t i u m atoms t o 4.5 double bond i n A -3-keto s t e r o i d o c c u r r e d s g i v i n g r i s e t o a mixture o f 5a-and 5Bisomers i n about equal q u a n t i t i e s .