Tritium labeling of peptides and proteins: A critical review
β Scribed by C.T. Peng; R.L. Hua
- Book ID
- 103114469
- Publisher
- Elsevier Science
- Year
- 1985
- Weight
- 56 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0020-708X
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In the interest of developing efficient methods for tritium labelling peptides, we here demonstrate the successful labelling of PACAPβ38 (pituitary adenylate cyclaseβactivating polypeptide), a 38βmer peptide, using a synthetic diiodinated PACAPβ38 precursor. In this example, we employ standard hydro
Optically pure L-valine was labelled with tritium by the Wilzbach method. Only 4 of the activity of the radiochemically pure valine was found in D-valine. This figure was determined by reverse isotope dilution. The suitability of this method for valine was tested, wich good results, with DL-valine