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Tritium isotope effect in high-pressure liquid chromatography of vitamin D metabolites

✍ Scribed by Graeme K. Worth; Robert W. Retallack

Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
391 KB
Volume
174
Category
Article
ISSN
0003-2697

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✦ Synopsis

A significant chromatographic isotope effect is reported for 1,25-dihydroxyvitamin D3 in a wide variety of HPLC separation systems. The effect is also observed for 24,25-dihydroxyvitamin D3. Retention times differ from less than 1% up to 4% depending on the separation system and the degree and position of tritium substitution. Such an effect must be corrected for whenever both labeled and unlabeled vitamin D metabolites are used in HPLC cochromatography or assay recovery studies.

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