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Analysis of vitamin A in eggs by high pressure liquid chromatography

✍ Scribed by Ötles, Semih ;Hisil, Yasar

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
185 KB
Volume
35
Category
Article
ISSN
0027-769X

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✦ Synopsis

Analysis of vitamin A in eggs by high pressure liquid chromatography

Semih o d e s and Yasar Hisil A high speed liquid chromatographic method was described for the rapid determination of vitamin A in eggs. After saponification and extraction, the sample was separated on a reversed-phase column (C18) with acetonitrilemethylene-chloridemethanol as the mobile phase. Quantitative determination was accomplished by monitoring the ultraviolet absorbance of retinol at 280 nm. Recovery of vitamin A was 97.1 %.

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