𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Analysis of enzymatic steroid conversions by high pressure liquid chromatography

✍ Scribed by S.A. Slocum; J.F. Studebaker

Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
271 KB
Volume
68
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

Enzyme catalyzed introduction of the 1-2 double bond into a steroid can be monitored through spectrophotometric changes accompanying electron acceptor reduction or through paper or thin-layer chromatographic analysis of the reaction product. The spectrophotometric method is not applicable to cases in which the oxidized form of the electron acceptor is continually regenerated. In studying such cases, we have found high pressure liquid chromatography (HPLC) to be a method of direct analysis more convenient than paper chromatography or tic. Use of a water based eluant and a reverse phase column for the HPLC analysis allows direct injection of a sample of the aqueous reaction solution after acidification, and no extraction with an organic solvent is necessary.

πŸ“œ SIMILAR VOLUMES

Analysis of cardenolides of Convallaria
Analysis of cardenolides of Convallaria majalis by high pressure liquid chromatography
✍ Harkiss, K. J. ;Linley, P. A. ;Mesbah, M. K. M. πŸ“‚ Article πŸ“… 1981 πŸ› John Wiley and Sons 🌐 English βš– 174 KB πŸ‘ 1 views

## Abstract Cardenolides of __Convallaria majalis__ were separated by reverse‐phase HPLC on Partisil PXS 10/25 ODS columns using 25% acetonitrile as mobile phase. Periplorhamnoside, convallatoxin and either convallatoxol or convalloside were resolved from glycoside mixtures. Convallatoxin and perip

Analysis of vitamin A in eggs by high pr
Analysis of vitamin A in eggs by high pressure liquid chromatography
✍ Γ–tles, Semih ;Hisil, Yasar πŸ“‚ Article πŸ“… 1991 πŸ› John Wiley and Sons 🌐 English βš– 185 KB πŸ‘ 1 views

## Analysis of vitamin A in eggs by high pressure liquid chromatography Semih o d e s and Yasar Hisil A high speed liquid chromatographic method was described for the rapid determination of vitamin A in eggs. After saponification and extraction, the sample was separated on a reversed-phase column