Enzyme catalyzed introduction of the 1-2 double bond into a steroid can be monitored through spectrophotometric changes accompanying electron acceptor reduction or through paper or thin-layer chromatographic analysis of the reaction product. The spectrophotometric method is not applicable to cases i
Analysis of steroid phosphates by high-pressure liquid chromatography: Betamethasone sodium phosphate
โ Scribed by L. M. Upton; E. R. Townley; F. D. Sancilio
- Publisher
- John Wiley and Sons
- Year
- 1978
- Tongue
- English
- Weight
- 424 KB
- Volume
- 67
- Category
- Article
- ISSN
- 0022-3549
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๐ SIMILAR VOLUMES
Quantitation of individual phospholipids sepamted by HPLC from tissue extracts by calorimetric analysis of phosphate was investigated. Elution of inorganic phosphate and breakthrough of lecithin were determined using radioisotopes. A substance which interfered with sample phosphate determinations wa
Compounds IVb-IVg were prepared similarly from I11 and the appropriate alcohols (Table I). p-Chlorophenyl Benzo[ b]-3-methyl-2-furancarbamate (1Vb)-A solution of I11 (2.01 g, 0.01 mole) and p-chlorophenol(l.28 g, 0.01 mole) in 30 ml of dry benzene was refluxed for 4 hr. The solvent was evaporated,
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