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High-performance liquid chromatography of phospholipids: Quantitation by phosphate analysis

✍ Scribed by John R. Yandrasitz; Gerard Berry; Stanton Segal

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
381 KB
Volume
135
Category
Article
ISSN
0003-2697

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✦ Synopsis

Quantitation of individual phospholipids sepamted by HPLC from tissue extracts by calorimetric analysis of phosphate was investigated. Elution of inorganic phosphate and breakthrough of lecithin were determined using radioisotopes. A substance which interfered with sample phosphate determinations was found in the column eluant, and a method to minimize its effect was developed. This method allows accurate quantitation of individual phospholipids present at a minimum of 20 nmol phosphate.

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