𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Separation and Quantitation of Phospholipids and Lysophospholipids by High-Performance Liquid Chromatography

✍ Scribed by Edward J. Lesnefsky; Maria S.K. Stoll; Paul E. Minkler; Charles L. Hoppel

Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
94 KB
Volume
285
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

We describe a comprehensive approach to the separation, quantitation, and characterization of phospholipids and lysophospholipids present in complex biological samples. The central feature is a normal-phase HPLC separation of individual phospholipid and lysophospholipid classes. In this single chromatographic step, phospholipids and lysophospholipids are separated and recovered for quantitation by organic phosphate assay and characterization by acyl-group composition. Recovery of phospholipids and lysophospholipids from HPLC averages 80 -90%. Isolated phospholipid and lysophospholipid fractions are available for separation of individual molecular species by second-dimension reverse-phase HPLC and characterization of individual molecular species by mass spectrometry.

πŸ“œ SIMILAR VOLUMES

Separation and Quantitation of Phospholi
Separation and Quantitation of Phospholipids and Their Ether Analogues by High-Performance Liquid Chromatography
✍ Zhizhong Guan; Jacob GrΓΌnler; Shengfu Piao; Pavel J. Sindelar πŸ“‚ Article πŸ“… 2001 πŸ› Elsevier Science 🌐 English βš– 77 KB

The common mobile phase hexane/isopropanol/water used for separation of phospholipids on highperformance liquid chromatography silica columns poses several problems, such as incomplete separation and rapid column deterioration. By inclusion of 5 mM ammonium sulfate in the aqueous phase, we were able

High-performance liquid chromatography o
High-performance liquid chromatography of phospholipids: Quantitation by phosphate analysis
✍ John R. Yandrasitz; Gerard Berry; Stanton Segal πŸ“‚ Article πŸ“… 1983 πŸ› Elsevier Science 🌐 English βš– 381 KB

Quantitation of individual phospholipids sepamted by HPLC from tissue extracts by calorimetric analysis of phosphate was investigated. Elution of inorganic phosphate and breakthrough of lecithin were determined using radioisotopes. A substance which interfered with sample phosphate determinations wa

Separation and quantitation of dolichyl
Separation and quantitation of dolichyl esters by high-performance liquid chromatography
✍ Johan Ericsson; Tadeusz Chojnacki; Gustav Dallner πŸ“‚ Article πŸ“… 1987 πŸ› Elsevier Science 🌐 English βš– 404 KB

An HPLC procedure for the isolation and quantitation of total and individual dolichyl esters in tissues has been developed. The purified lipid extracts are subjected to sequential reversed-phase, straight-phase, and reversed-phase HPLC, which yield complete resolution and high recovery of the indivi

Chiral separation and quantitation of do
Chiral separation and quantitation of dorzolamide hydrochloride enantiomers by high-performance liquid chromatography
✍ Mojtaba Shamsipur; Rouhollah Heydari; Elahe Sadat Jamasbi; Assem Abdollahpour πŸ“‚ Article πŸ“… 2010 πŸ› John Wiley and Sons 🌐 English βš– 186 KB πŸ‘ 1 views

## Abstract A direct HPLC method for chiral separation of dorzolamide hydrochloride (4__S__,6__S__) and its enantiomer (4__R__,6__R__) was developed. Dorzolamide (4__S__,6__S__) and its antipode were separated on a chiral‐α~1~‐acid glycoprotein column (150Γ—4.0 mm, 5 μm). The influences of pH, tempe

High-Performance Liquid Chromatography S
High-Performance Liquid Chromatography Separation and Quantitation of Methylprednisolone from Rat Brain
✍ E.J. Murphy; A.P. Slivka; T.A. Rosenberger; L.A. Horrocks πŸ“‚ Article πŸ“… 1993 πŸ› Elsevier Science 🌐 English βš– 313 KB

A sensitive, reliable method for the extraction, separation, and quantitation of methylprednisolone from rat brain is reported. The method can accurately quantitate methylprednisolone levels between 9.8 and 2500 ng/injection using a two-step HPLC separation and monitoring absorbance at 254 nm. A 90%