High Performance Liquid Chromatographic Analysis of Soybean Phospholipids

✍ Scribed by Sotirhos, N. ;Ho, Chi-Tang ;Chang, Stephen S.

• Publisher: John Wiley and Sons
• Year: 1986
• Weight: 350 KB
• Volume: 88
• Category: Article
• ISSN: 0931-5985
• DOI: 10.1002/lipi.19860880103

✦ Synopsis

High Performance Liquid Chromatographic Analysis

of Soybean Phospholipids *

B y N . S o t i r h o s , C h i -T a n g H o andStephenS. C h a n g *

An HPLC method was optimized to analyze soybean phospholipids. It uses a silica column with W detection at 210 nm, and amobile phase consisting ofhexane,isopmpanol and water. Separation isachieved by keeping the hexane toisopropanolratioconstant andvaryingthewaterconcentrationina gradient elution program. Components in the HPLC chromatogram were identified by comparison with retention times of authentic compounds.The