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Transplantable mouse tumor line induced by injection of SV40-transformed mouse kidney cells

✍ Scribed by Saul Kit; T. Kurimura; D. R. Dubbs

Publisher
John Wiley and Sons
Year
1969
Tongue
French
Weight
615 KB
Volume
4
Category
Article
ISSN
0020-7136

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✦ Synopsis

A transplantable tumor of inbred mice was obtained by inoculating BALBIc mice subcutaneously with S V40-transformed mouse kidney (mKS-A) cells. Tumors were produced by mKS-A cells in the 7lst cell culture passage, but not by cells in the 26th passage. The tumor line has been serially passed in BALBIc mice 14 times. In vitro cell culture lines were derived from tumors after I , 2, 8, I0 and 12 passages in mice. The tumors, as well as the in vitro tumor cell lines, contained S V40 T-antigen, and sera from the tumor-bearing mice contained antibodies to the S V40 T-antigen. S V40 was rescued from the in vitro tumor cell lines after fusion with green monkey kidney ( C V -I ) cells in the presence of UV-irradiated Sendai virus.

The in vitro tumor cell lines derived from mouse passages 8, I0 and 12 were used as SV40 virus; 2 ) SV4O-transformed cell lines; 3 ) primary mouse (BALBIc or Yale Swiss) kidney cells, or 4 ) primary mouse (BALBIc or Yale Swiss )embryo cells. These results showed that the tumor line and the in vitro tumor cell lines have the transplantation antigen.

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