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Isolation and characterization of revertant cell lines. IV. Direct selection of serum-revertant sublines of SV40-transformed 3T3 mouse cells

✍ Scribed by A. Vogel; R. Pollack

Publisher
John Wiley and Sons
Year
1973
Tongue
English
Weight
698 KB
Volume
82
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

SV101, the SV40‐transformed subline of the mouse fibroblast line 3T3, is both serum‐ and density transformed, since it grows in both 1% and 10% calf serum, and grows beyond confluence in 10% calf serum. Negative selection at low cell density in 1% calf serum or in 10% agamma‐depleted serum permits direct recovery of serum‐revertant sublines of SV101. These sublines are unable to grow in 1% calf serum.

Although negative selection at high cell density in 10% calf serum is known to permit recovery of density‐revertant sublines of SV101, most density‐revertants are not serum‐revertant. However, all serum‐revertants isolated so far are density‐revertant as well.

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