Surface glycoproteins and concanavalin-A-mediated agglutinability of clonal variants and tumour cells derived from SV40-virus-transformed mouse 3T3 cells

✍ Scribed by L. A. Smets; W. P. Van Beek; H. Van Rooij

• Publisher: John Wiley and Sons
• Year: 1976
• Tongue: French
• Weight: 538 KB
• Volume: 18
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910180411

✦ Synopsis

Abstract

Cell strains isolated from an established line of SV40‐transformed mouse 3T3 cells (SV3T3) showed large variations in the various parameters of transformation, viz. saturation density, serum requirement, contact inhibition of movement and of growth and Concanavalin‐A‐mediated agglutinability. These cell strains were studied for changes in elution profiles of fucose‐labelled surface glycoproteins, using actively growing, untransformed 3T3 cells as controls. A cell strain established from a tumour arising after injection of wild‐type SV3T3 cells and SV3T3 cells grown in vitro in diffusion chambers, was similarly studied. Changes in surface glycoproteins were observed only in the tumour‐derived cell strain. The results suggest that alterations in surface glycoproteins are associated with the tumorigenic potential of the cells rather than with the transformed phenotype per se.