𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Isolation and characterization of revertant cell lines. II. Growth control of a polyploid revertant line derived from SV40-transformed 3T3 mouse cells

✍ Scribed by Robert Pollack; Arthur Vogel

Publisher
John Wiley and Sons
Year
1973
Tongue
English
Weight
610 KB
Volume
82
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

3T3 mouse fibroblasts in 10% calf serum cease to increase in number at confluence. SV40‐transformed 3T3 cells lose their sensitivity to this growth control and so are able to reach multilayered cell densities. We previously described a polyploid revertant line F1SV101, isolated from a SV40‐transformed cell, that has a low maximum cell density (Pollack et al., '68), despite the continued presence in this revertant of SV40‐specific DNA, RNA and Tantigen. We have examined the mechanism by which the revertant maintains a low saturation density, and find that at confluence the revertant and 3T3 are both reduced in mitotic index, fraction of cells synthesizing DNA, and rate of DNA synthesis. The transformed cell does not respond to confluence. None of the lines sheds intact cells into the medium at confluence.

📜 SIMILAR VOLUMES

Isolation and characterization of revert
Isolation and characterization of revertant cell lines. IV. Direct selection of serum-revertant sublines of SV40-transformed 3T3 mouse cells
✍ A. Vogel; R. Pollack 📂 Article 📅 1973 🏛 John Wiley and Sons 🌐 English ⚖ 698 KB

## Abstract SV101, the SV40‐transformed subline of the mouse fibroblast line 3T3, is both serum‐ and density transformed, since it grows in both 1% and 10% calf serum, and grows beyond confluence in 10% calf serum. Negative selection at low cell density in 1% calf serum or in 10% agamma‐depleted se

Isolation and characterization of revert
Isolation and characterization of revertant cell lines. III. Isolation of density-revertants of SV40-transformed 3T3 cells using colchicine
✍ A. Vogel; R. Risser; R. Pollack 📂 Article 📅 1973 🏛 John Wiley and Sons 🌐 English ⚖ 602 KB

## Abstract Treatment of the SV40 transformed 3T3 cell line SV101 with colchicine permits the isolation of polyploid revertant sublines Which have lower saturation densities than SV101. These low saturation density lines have also reverted to a high serum requirement for growth, and are unable to f

The characterization of SV40-transformed
The characterization of SV40-transformed cell lines derived from mouse teratocarcinoma: Growth properties and differentiated characteristics
✍ W. Topp; J. D. Hall; D. Rifkin; A. J. Levine; R. Pollack 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 English ⚖ 992 KB

## Abstract Mouse teratocarcinoma cells derived from embryoid bodies of 129SVsl mice were cultured in vitro to permit their differentiation. These cells were then infected with simiam virus 40 (SV40) and 31 cloned cell lines (SVTER) were derived from these cultures. All 31 SVTER cell lines containe

Surface glycoproteins and concanavalin-A
Surface glycoproteins and concanavalin-A-mediated agglutinability of clonal variants and tumour cells derived from SV40-virus-transformed mouse 3T3 cells
✍ L. A. Smets; W. P. Van Beek; H. Van Rooij 📂 Article 📅 1976 🏛 John Wiley and Sons 🌐 French ⚖ 538 KB

## Abstract Cell strains isolated from an established line of SV40‐transformed mouse 3T3 cells (SV3T3) showed large variations in the various parameters of transformation, viz. saturation density, serum requirement, contact inhibition of movement and of growth and Concanavalin‐A‐mediated agglutinab