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Enhancing effect of green monkey kidney cell extract on the transformation of mouse cells by SV40

✍ Scribed by Akio Hagiwara; Tsunehiro Kitahara

Publisher
John Wiley and Sons
Year
1974
Tongue
French
Weight
450 KB
Volume
14
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

Extracts of green monkey kidney cells enhance the transformation of mouse cells by SV40. An enhancing activity is also detected in cell extracts from human embryonic lung, mouse embryo or hamster embryo cells. The activity of green monkey kidney cell extract is sensitive to trypsin, but not deoxyribonuclease, ribonuclease or heating at 56° C for 30 min. Since the extract has no effect on early events such as virus adsorption, penetration, uncoating and T antigen formation, the enhancing factor(s) may act on the other processes required for transformation.

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