Transforming growth factor beta 1 (TGF-β1) receptor expression on resting and mitogen-activated T cells

✍ Scribed by Larry Ellingsworth; Debra Nakayama; James Dasch; Patricia Segarini; Pedro Carrillo; Wendy Waegell

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 693 KB
• Volume: 39
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.240390414

✦ Synopsis

Transforming growth factor Pl (TGF-01) is a potent autocrine growth inhibitor of lymphocytes. In this study, the expression of TGF-01 binding proteins was characterized on murine splenic T cells. With an affinity cross-linking method and by neutralizing antibodies to TGF-P1, [I2'I] TGF-P1 was found to bind to three cell surface-binding proteins (280-200 kD, 95-85 kD, 65 kD) that were differentially expressed on resting and mitogen-stimulated T cells. Freshly prepared (resting) T cells were found to constitutively express the 95-85-kD form of these binding proteins, whereas mitogenic stimulation by either concanavalin-A (Con-A), interleukin-1 (IL-l), interleukin-2 (IL-2), or 12-tetradencanoyl-phorbol-13-acetate (TPA) for 12-72 h induced the appearance of all forms of the TGF-P1 binding proteins (280-200 kD, 95-85 kD, and 65 kD). Furthermore, antibodies that neutralized the biologic action of TGF-j31 also blocked the binding of ["'I] TGF-P1 to all three binding proteins, suggesting that these binding proteins are involved with signal transduction. These results suggest that the expression of the TGF-P1 receptor on T cells is regulated by T cell mitogenic signals and that a regulatory relationship may exist between T cell growth-promoting cytokines (IL-1 and IL-2) and the T cell growth inhibitor, TGF-01.