Expression of transforming growth factor-beta (TGF-β) receptors, TGF-β1 and TGF-β2 production and autocrine growth control in osteosarcoma cells

✍ Scribed by Peter Kloen; Candace L. Jennings; Mark C. Gebhardt; Dempsey S. Springfield; Henry J. Mankin

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: French
• Weight: 778 KB
• Volume: 58
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910580323

✦ Synopsis

Transforming growth factor-beta (TGF-P) is a polypeptide with multiple physiological functions. lsoforms of this growth factor have important roles in control of the cell cycle, in regulation of cell-cell interactions and in growth and development. Malignant transformation has been shown to be associated with increased expression of TGF-P. Since bone is the largest storage site and producer of TGF-P, we speculated on the existence of an autocrine mechanism in osteosarcoma, a malignant bone tumor. Expression of TGF-P cell surface receptors, effects on growth of TGF-P and TGF-p antibodies and production of 2 TGF-P isoforms were studied in a panel of 7 osteosarcoma cell lines. In contrast to most previous reports on the effects of TGF-P on osteosarcoma cell growth, we found a mitogenic effect of TGF-PI in 4 of 7 osteosarcoma cell lines. Receptor profiles for TGF-P were aberrant in 5 of the 7 cell lines tested, and production of TGF-P I and TGF-P2 varied among cell lines. Addition of anti-TGF-fJ antagonized the effects of endogenous TGF-P. Our results suggest a potential role of TGF-P in autocrine growth control of osteosarcoma cells.