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Transformation ofPenicillium chrysogenumusing theAspergillus nidulans amdSgene as a dominant selective marker

✍ Scribed by Raj K. Beri; Geoffrey Turner

Book ID
104753945
Publisher
Springer-Verlag
Year
1987
Tongue
English
Weight
251 KB
Volume
11
Category
Article
ISSN
0172-8083

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✦ Synopsis

The Aspergillus nidulans acetamidase gene (amdS) has been used to transform Penicillium chrysogenum at low frequency. Several transformants were tested and shown to be mitotically stable. Southern blot analysis indicated that transforming DNA had integrated into the chromosomal DNA, possibly at multiple sites.

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