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Zeocin resistance as a dominant selective marker for transformation and targeted gene deletions in Candida glabrata

✍ Scribed by Alex J. Alderton; Ian Burr; Fritz A. Mühlschlegel; Mick F. Tuite


Book ID
108914304
Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
192 KB
Volume
49
Category
Article
ISSN
0933-7407

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A transformation system using resistance to the antibiotic cycloheximide as a dominant selectable marker was developed for the pathogenic yeast Cryptococcus neoformans. A 3.5 kb DNA fragment containing a gene encoding the ribosomal protein L41 was cloned from a wild-type strain of C. neoformans whic