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Cloning of the nitrate reductase gene of Stagonospora (Septoria) nodorum and its use as a selectable marker for targeted transformation

โœ Scribed by S. B. Cutler; R. Neil Cooley; Christopher E. Caten


Publisher
Springer-Verlag
Year
1998
Tongue
English
Weight
312 KB
Volume
34
Category
Article
ISSN
0172-8083

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A transformation system using resistance to the antibiotic cycloheximide as a dominant selectable marker was developed for the pathogenic yeast Cryptococcus neoformans. A 3.5 kb DNA fragment containing a gene encoding the ribosomal protein L41 was cloned from a wild-type strain of C. neoformans whic