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Transformation ofPenicillium chrysogenumwith a dominant selectable marker

✍ Scribed by John H. Bull; David J. Smith; Geoffrey Turner

Book ID
104741891
Publisher
Springer-Verlag
Year
1988
Tongue
English
Weight
556 KB
Volume
13
Category
Article
ISSN
0172-8083

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✦ Synopsis

We have cloned a mutant oligomycin resistance allele of the mitochondrial ATP synthase subunit 9 gene from the filamentous fungus Penicillium chrysogenum. The gene was isolated using the equivalent gene from Aspergillus nidulans as a hybridisation probe. Using the cloned gene it is possible to select for oligomycin resistance in P. chrysogenum transformation experiments. This transformation system was used to introduce further copies of the P. chrysogenum isopenicillin N synthetase gene, which were stably maintained without selection. An assessment of the frequency with which homologous integration occurs was also made. With this system, it should prove possible to transform any strain of P. chrysogenum.

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