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Use of mutatedPDR3gene as a dominant selectable marker in transformation of prototrophic yeast strains

✍ Scribed by D. Lacková; J. Šubík


Publisher
Springer Netherlands
Year
1999
Tongue
English
Weight
613 KB
Volume
44
Category
Article
ISSN
0015-5632

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A transformation system using resistance to the antibiotic cycloheximide as a dominant selectable marker was developed for the pathogenic yeast Cryptococcus neoformans. A 3.5 kb DNA fragment containing a gene encoding the ribosomal protein L41 was cloned from a wild-type strain of C. neoformans whic