𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Transformation of undomesticated strains of Bacillus subtilis by protoplast electroporation

✍ Scribed by Diego Romero; Alejandro Pérez-García; Jan-Willem Veening; Antonio de Vicente; Oscar P. Kuipers

Book ID
116750053
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
91 KB
Volume
66
Category
Article
ISSN
0167-7012

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

High frequency transformation of Bacillu
High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA
✍ Chang, Shing ;Cohen, Stanley N. 📂 Article 📅 1979 🏛 Springer 🌐 English ⚖ 449 KB

A highly efficient method for transformation of Bacillus subtilis by plasmid DNA is reported. The procedure, which involves polyethylene glycol-induced DNA uptake by protoplasts and subsequent regeneration of the bacterial cell wall, yields up to 80% transformants with an efficiency of 4 x 10(7) tra