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Transformation of barley scutellum protoplasts: regeneration of fertile transgenic plants

โœ Scribed by J. Nobre; M. R. Davey; P. A. Lazzeri; M. E. Cannell

Book ID
106010451
Publisher
Springer
Year
2000
Tongue
English
Weight
156 KB
Volume
19
Category
Article
ISSN
0721-7714

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Regeneration of fertile plants from embryogenic suspension culture protoplasts ofSorghum vulgare
โœ Zhi-ming Wei; Zhi-hong Xu ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Springer ๐ŸŒ English โš– 550 KB

Protoplasts were isolated from immature inflorescence-derived embryogenic suspension cultures of two cultivars of Sorghum vulgare. The protoplasts were cultured in a modified K8P liquid medium. They started to divide after 4-5 days of culture, and achieved 16.8% division frequency by 10 days. Protoc